EZ Cap™ Mouse RLI mRNA (m1Ψ)

RLI, a fusion protein composed of interleukin-15 (IL-15) and the sushi domain of its receptor IL-15Rα, is an innovative immunotherapeutic agent. As an important cytokine, IL-15 has the ability to stimulate the proliferation of natural killer cells (NK cells) and T cells, thereby enhancing the anti-tumor and antiviral response of the immune system. However, the biological activity of IL-15 depends on binding to its receptor IL-15Rα. By fusing IL-15 with the sushi domain of IL-15Rα, the resulting fusion protein RLI stabilizes the activity of IL-15 and prolongs its half-life in vivo. This design not only improves the bioavailability of IL-15, but also reduces the toxicity that may be induced by IL-15 alone. IL-15/IL-15Rα-sushi fusion protein has shown potential in cancer immunotherapy to enhance the function of immune cells and promote the clearance of tumor cells. Studies have shown that this fusion protein has significant antitumor activity in a variety of cancer models, and has become an important research direction in the field of immunotherapy.

EZ Cap™ Mouse RLI mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.