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Biotin-XX Tyramide Reagent

Catalog No.
A8012
Reagent used for tyramide signal amplification (TSA)
Grouped product items
SizePriceStock Qty
10mg
$100.00
In stock
100mg
$700.00
In stock
1g
$2,200.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Biotin-LC-LC-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-LC-LC-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt589.79
FormulaC30H47N5O5S
Synonymsbiotin-LC-LC-tyramide
Solubility≥59 mg/mL in DMSO, ≥14.1 mg/mL in EtOH with ultrasonic, insoluble in H2O
Chemical NameN-(4-hydroxyphenethyl)-6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamide
SDFDownload SDF
Canonical SMILESO=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCCCCC(NCCCCCC(NCCC3=CC=C(O)C=C3)=O)=O)=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

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