EZ Cap™ Mouse IFN-γ mRNA (5mCTP, ψUTP)

Interferon gamma (IFN-γ) is an important cytokine secreted mainly by activated T lymphocytes and natural killer (NK) cells. It plays a key role in the immune system, and its main functions include activating macrophages and improving their ability to engulf and kill pathogens. In addition, IFN-γ also promotes the function of antigen-presenting cells, enhancing the recognition of antigens and tumor cells by T cells through increased expression of major histocompatibility complex (MHC) molecules. IFN-γ also plays an important role in regulating Th1 immune response and inhibiting Th2 immune response, which plays an important role in maintaining immune balance.

EZ Cap™ Mouse IFN-γ mRNA (5mCTP, ψUTP) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of 5mCTP/ψUTP and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.