EZ Cap™ CPF1/Cas12a mRNA (m1Ψ)

Cpf1 (also known as Cas12a) is a nuclease in the CRISPR system. Compared with Cas9, Cpf1 has unique advantages: it recognizes PAM sequences rich in T, generating DNA incisions with sticky ends, which is convenient for gene editing. Cpf1 mRNA can be introduced into cells to express Cpf1 protein, and then combine with guide RNA (crRNA) to form the CPF1-crRNA complex, which precisely recognizes and cleases the target DNA sequence. Cpf1 has broad application prospects in fields such as gene editing, disease treatment and agricultural breeding.

EZ Cap™ CPF1/Cas12a mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It expressed Cpf1 protein. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.