Cell Proliferation

Overview

Understanding the dynamics of cell growth, division, and survival is fundamental to life science research and drug discovery. Our comprehensive portfolio of advanced tools is designed to deliver accurate, sensitive, and reliable data for your cell proliferation and viability studies. Whether you are performing high-throughput screening, kinetic monitoring of live cells, or detailed mechanistic analysis, we provide robust solutions—from classic endpoint assays to real-time technologies. Accelerate your research with our trusted reagents, optimized protocols, and integrated platforms to gain deeper insights into cellular health, compound toxicity, and therapeutic mechanisms.

Cell Proliferation Assays Selection

Method	Maker	Benefits	Limitations
Metabolism Activity	MTT XTT MTS CCK-8 Simple and cost-effective Well-suited for high-throughput screening Dependent on metabolic activity, not direct proliferation Activity variable under different conditions Resazurin Low toxicity and inexpensive High sensitivity Supports long-term monitoring Dependent on metabolic activity, not direct proliferation Signal sensitive to pH, oxygen, and redox agents
DNA synthesis	BrdU Directly labels newly synthesized DNA Signal scales with proliferation Compatible with microscopy and flow cytometry Requires harsh DNA denaturation Not suitable for multiplex staining Lower sensitivity and higher background Time-consuming procedure EdU Higher selectivity compared to BrdU Simple, rapid, and highly sensitive Low toxicity Allows for multiplexing More expensive than BrdU Limited to proliferating cells with EdU uptake capability
ATP Assay	Simply, highly sensitive Ultra-fast results Well-suited for high-throughput screening Measures metabolic activity, not direct proliferation Require cell lysis Relatively expensive
Proliferation Maker	Ki67 Direct marker for active proliferation Widely used in tissue samples Compatible with multiplexing Subjective in quantification Requires antibodies Time-consuming procedure PCNA Robust marker for DNA replication Established, widely-used protocol in histology Subjective in quantification Requires antibodies Time-consuming procedure
Dye Dilution Assay	CFSE Long-term tracing of cellular proliferation Compatible with flow cytometry Highly susceptible to hydrolysis High dye concentration may inhibit proliferation

Metabolism Activity

Measures cell viability and proliferation by quantifying the metabolic activity of live cells. Simple, widely used, suitable for microplate reader, and scalable for high-throughput screening.

Products	Catalog No.	Features
MTT Cell Proliferation Assay Kit	K2249 Absorbance at 570 nm Classic formazan-based assay, simple and inexpensive Requires cell lysis but no organic solvents
MTS Cell Proliferation Assay Kit K2250 Fluorescence at 590/617 nm XTT Cell Proliferation Assay Kit K2272 Fluorescence at 646/662 nm		Higher sensitivity than MTT No lysis step required Requires pre-mixing before use
Cell Counting Kit-8 (CCK-8)	K1018 Absorbance at 450 nm Higher sensitivity than MTT, MTS, XTT Ready-to-use solution, no-toxic Simple, rapid procedure
Cell Counting Kit-8 (CCK-8) Plus	K2268 Absorbance at 450 nm Enhanced sensitivity vs. standard CCK-8 Ideal for high-throughput screening
Resazurin Cell Viability Assay Kit	K2234 Fluorescence at 570/595 nm Fluorescent readout of metabolic activity Non-toxic to cells, ready-to-use
Calcein AM Cell Viability Assay Kit	K2231 Fluorescence at 494/517 nm Detects viable cells via esterase activity

Validation

DNA Synthesis

Detects DNA synthesis in proliferating cells using a thymidine analog. Highly sensitive and rapid, this series covers applications from flow cytometry and fluorescence microscopy to optical imaging and microplate detection.

Products	Catalog No.	Detection	Features
EdU Imaging Kits (FITC) K1175 EdU Imaging Kits (HF488) K2240 Fluorescence at 496/516 nm EdU Imaging Kits (Cy3) K1075 Fluorescence at 555/570 nm EdU Imaging Kits (HF594) K2243 Fluorescence at 590/617 nm EdU Imaging Kits (Cy5) K1076 Fluorescence at 646/662 nm			Suitable for detection by fluorescence microscopy and flow cytometry Especially optimized for microscopy
EdU Flow Cytometry Assay Kits (FITC) K1176 EdU Flow Cytometry Assay Kits (HF488) K2241 Fluorescence at 496/516 nm EdU Flow Cytometry Assay Kits (Cy3) K1077 Fluorescence at 555/570 nm EdU Flow Cytometry Assay Kits (HF594) K2244 Fluorescence at 590/617 nm EdU Flow Cytometry Assay Kits (Cy5) K1078 Fluorescence at 646/662 nm			Optimized for flow cytometry
EdU Imaging Kits (DAB) K2257 Optical imaging			Optimized for light microscope
EdU Cell Proliferation Kit (TMB) K2279 Absorbance at 370 nm or 620-650 nm			Optimized for microplate reader
5-Ethynyl-2'-deoxyuridine (5-EdU) B8337 -			High-purity compounds for animal experiments

Validation

ATP Assay

Quantifies intracellular ATP as a direct indicator of metabolically active cells. It is highly sensitive and provides results in as little as 10 minutes, making it ideal for rapid proliferation and cytotoxicity screening.

Products	Catalog No.	Detection	Features
Luminescent ATP Cell Viability Assay Kit I K2041 Luminescent ATP Cell Viability Assay Kit II K2042		Luminescence	Ready-to-use, simple Lyophilized format for enhanced stability

Validation

Proliferation Maker

Uses antibodies to detect specific proteins expressed during active phases of the cell cycle. Ideal for immunohistochemistry, imaging, and flow cytometry.

Products	Catalog No.	Target	Application
Anti-Ki67 Rabbit Monoclonal Antibody	MA3407	Human	IHC-P
Anti-Ki67 Rabbit Monoclonal Antibody	MA3406	Human	WB, IHC-P, ICC/IF, FC
Anti-PCNA (6B12) Mouse Monoclonal Antibody	MA5776	Human, Mouse, Monkey, Rat, Hamster	WB, ICC/IF, IP
Anti-PCNA (9C9) Mouse Monoclonal Antibody	MA5777	Human, Mouse, Rat	WB, IHC-P
Anti-PCNA (2E1) Mouse Monoclonal Antibody	MA5787	Human, Mouse, Monkey, Rat, Hamster	WB, IHC-F, IHC-P, ICC/IF, IP
Anti-PCNA Rabbit Monoclonal Antibody	MA5799	Human, Mouse	WB
Anti-PCNA Rabbit Monoclonal Antibody	MA5800	Human, Mouse, Rat	WB, ICC/IF, IP

Dye Dilution Assay

Tracks cell division by measuring the progressive dilution of a fluorescent dye in daughter cells. Perfect for long-term proliferation studies and flow cytometry analysis.

Products	Catalog No.	Detection	Features
CFDA SE Cell Tracer Kit	K1023	Fluorescence at 494/521 nm	A classic and widely used tracer dye Bright, stable signal Compatible with flow cytometry
CellTracker Red CMTPX	B8827	Fluorescence at 577/602 nm	Cell-permeant red fluorescent probe Stable signal with low phototoxicity Compatible with multiplexing & live imaging

Cell Proliferation

Overview