EdU Flow Cytometry Assay Kits (Cy5)

Measuring cell proliferation and cell cycle are a fundamental method to assess cell health, determine genotoxicity, and evaluate drug’s pharmacodynamic effect. The common method is measuring DNA synthesis directly. In previous experiments, there are several approaches such as the incorporation of radioactive nucleosides (3H-thymidine) or BrdU. Here, we introduce one new method, click chemistry-CuAAC (Copper-Catalyzed Azide-Alkyne Cycloaddition), and the use of this reaction in direct measurement of S-phase DNA synthesis in cell cycle.

A nucleoside analog of thymidine, EdU (5-ethynyl-2’-deoxyuridine), can be incorporated into DNA strand during DNA synthesis. The alkynyl group of EdU is a biologically inert group that will undergo an extremely selective reaction with dye’s azido via a CuAAC reaction to afford an 1,2,3-triazole product. EdU and Cy5 azide possess biologically unique moieties to label DNA of proliferating cells, producing low backgrounds and high detection sensitivities. This CuAAC reaction affords superior regioselectivity and quantitative transformation under extremely mild conditions.

EdU Flow Cytometry Assay Kits (Cy5) is a sensitive and reliable way to detect and quantify cell proliferation in cells. It is optimized for flow cytometry.

Simple works in less time with no denaturation steps or harsh treatment

High efficiency with robust brightness

Compatible with some antibodies and cell cycle dyes

High consistency because of independence of variable anti-BrdU antibody