5-BrdU
5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.
In vitro: The effect of 5-BrdU on the proliferation was studied in the early phases of dedifferentiation of Nicotiana glauca pith explants grown. It was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].
In vivo: Young adult offspring mice were stained for 5-BrdU using a monoclonal antibody and the peroxidase-antiperoxidase method. The distribution of immunoreactive nuclei was very similar to the previous [3H] thymidine data. The pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. These data indicate that BrdU can be used to map neuronal birthdates [2].
Clinical trials: Currenlty no clinical data are available.
References:
[1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53-63
[2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1; 49 (2): 311-7.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 307.1 |
| Cas No. | 59-14-3 |
| Formula | C9H11BrN2O5 |
| Solubility | insoluble in EtOH; ≥11.56 mg/mL in 0.9% NS with ultrasonic; ≥15.35 mg/mL in DMSO; ≥16.23 mg/mL in H2O with ultrasonic |
| Chemical Name | 5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione |
| SDF | Download SDF |
| Canonical SMILES | BrC(C(N1)=O)=CN(C1=O)[C@@]2([H])C[C@@](O)([H])[C@@](O2)([H])CO |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Vitro experiment [1]: | |
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Samples |
Nicotiana pith tissues |
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Preparation method |
The solubility of this compound in DMSO is >15.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
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Reacting condition |
5 μg/mL; 24 h |
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Applications |
In nicotiana pith tissues, BrdU inhibited growth of pith explants, while thymidine completely reversed the inhibitory effect. The inhibition observed could be attributed to BrdU incorporation into DNA. |
| Animal experiment [2]: | |
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Animal models |
Pregnant NMRI mice |
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Dosage form |
25 mg/kg (5 mg/mL dissolved in 0.1 M Tris-buffered saline, pH 7.6), i.p.; between embryonic days E11 and E17. |
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Application |
In pregnant NMRI mice, a single pulse of 5-bromodeoxyuridine (BrdU) was injected between embryonic days E11 and E17 to label postmitotic neuroblasts. BrdU is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by polyclona or monoclonal antibodies. Immunoreactive nuclei were detected in the adult CNS after pulses of BrdU. BrdU could be used to map neuronal birthdates. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63 [2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1; 49 (2): 311-7. |
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