4-Thio-UTP
Catalog No.
B7978
A nucleoside triphosphate analog with oxygen at the 4-position of uracil substituted by sulfur, commonly used in UV cross-linking studies.
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4-Thio-UTP is a nucleotide triphosphate analog in which the oxygen atom at the 4-position of uracil is substituted by a sulfur atom. It can be incorporated into RNA molecules during in vitro transcription reactions, producing RNAs containing 4-thiouridine modifications. This modification provides unique advantages in studying protein interactions, RNA modifications, and RNA labeling. Due to the presence of the sulfur atom, RNA modified with 4-Thio-UTP can form cross-links with proteins upon ultraviolet (UV) irradiation, facilitating the study of RNA-protein interactions. Additionally, 4-Thio-UTP can be employed in research on RNA folding and function, as well as in studying the cellular localization and roles of RNA.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 500.2 (free acid) |
| Formula | C9H15N2O14P3S (free acid) |
| Synonyms | 4-Thiouridine-5'-Triphosphate, Thio UTP |
| Chemical Name | lithium (3,4-dihydroxy-5-(2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl triphosphate |
| SDF | Download SDF |
| Canonical SMILES | S=C(C=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| In Vitro Experiments [1] | |
| In vitro transcription assays and photoaffinity labelling of the transcriptional complex. | |
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Methods |
In vitro transcription reactions were carried out using RNA polymerase purified from lysed pea chloroplasts. The enzyme was incubated with the template DNA (5A/30T) containing the 16S rRNA promoter and a-32P-ATP (30 μCi, sp. act. 3000 Ci/mmole) and the photoprobe 4-thio UTP (200 μM) in the presence of 50mM Tris-HCl, pH 8.0, 10mM DTT, 1 μg BSA and 5mM MgCl2 at 25°C for 45 min. |
| 4-Thiouridine-5'-Triphosphate, also known as 4-Thio-UTP, is a potent agonist for P2Y2 and P2Y4 receptors. It is used in the study of crosslinking experiments, labeling of transcriptional complex. It also affects elongation and termination activity, causing transcriptional pause. |
Quality Control & MSDS
- View current batch:
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Purity = ≥90% by AX-HPLC
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
