IL-4, murine recombinant
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IL-4 is a pleiotropic cytokine produced by activated T lymphocytes, basophils, mast cells and bone marrow stromal cells. IL-4 plays an important role in the regulation of isotype switching, differentiation of precursor T helper cells and induction of IgE production in B lymphocytes. Also, IL-4 regulates cell proliferation, survival and gene expression. Excessive IL-4 production by Th2 cells is associated with elevated IgE production and allergy.
The murine IL-4 cDNA encodes a 140 aa protein with a 20 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific. Recombinant murine IL-4 is a 13.5 kDa globular protein containing 120 amino acid residues.
|Determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells.|
|ED50||< 2.0 ng/ml.|
|Specific activity||> 5*105 units/mg.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level: <0.1 ng/μg
|Gene ID:||16189||Accession #:||P07750|
|Alternate Names:||IL4, BCGF,BCDF, B cell stimulating factor, BSF-1,Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.|
|Source:||Escherichia Coli.||M.Wt:||13.5 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Lyophilized with no additives.|
|Solubility:||Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|