Recombinant Mouse IL-13, Tag Free
Interleukin-13 (IL-13) , is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils [1 - 3]. IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration [4, 5]. Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat [6, 7]. IL-13 has diverse activities on numerous cell types [8]. On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells.
Reference
[1]. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
[2]. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
[3]. Brown, K.D. et al. (1989) J. Immunol. 142:679.
[4]. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
[5]. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
[6]. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
[7]. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
[8]. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
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Accession # |
P20109 |
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Alternate Names |
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13 |
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Source |
Human embryonic kidney cell, HEK293-derived mouse IL-13 protein |
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Protein sequence |
Ser26-Phe131 |
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M.Wt |
11.5 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 years from date of receipt, -20 to -70°C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 0.5-1.5 ng/mL. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.