HyperScript™ IV RT SuperMix for qPCR

HyperScript™ IV Reverse Transcriptase is a genetically engineered fourth-generation reverse transcriptase based on M-MLV and provides superior robustness and reliability in RT reactions. The enzyme has significant improvements in inhibitor resistance, processivity, and reaction speed while retaining all the advantages of HyperScript™ III Reverse Transcriptase, including thermostability, highly efficient full-length cDNA synthesis, and reduced RNase H activity. The product can still provide reliable, consistent, and rapid cDNA synthesis in the presence of inhibitors (residues from RNA extraction). 

The HyperScript™ Ⅳ RT SuperMix for qPCR is designed for two-step RT-qPCR assays, 5 × HyperScript™ Ⅳ RT SuperMix contains all components for reverse transcription except template RNA. The maximum volume of the RNA template can be up to 80% of the total volume, making the kit ideal for reverse transcription of low concentrations RNA templates.

This product has been optimized for qPCR, especially the ratio of Oligo (dT)₂₃VN primer /Random primer, the cDNA synthesis can be initiated from all regions of the RNA transcript with the same efficiency, ensuring maximum authenticity and reproducibility of qPCR results. The products can be employed in SYBR Green or probe-based qPCR, you can choose appropriate reagents for gene expression analysis according to your purpose.