HyperScript™ IV RT SuperMix for qPCR (with gDNA wiper)

HyperScript™ IV Reverse Transcriptase is a genetically engineered fourth-generation reverse transcriptase based on M-MLV and provides superior robustness and reliability in RT reactions. The enzyme has significant improvements in inhibitor resistance, processivity, and reaction speed while retaining all the advantages of HyperScript™ III Reverse Transcriptase, including thermostability, highly efficient full-length cDNA synthesis, and reduced RNase H activity. The product can still provide reliable, consistent, and rapid cDNA synthesis in the presence of inhibitors (residues from RNA extraction).

The HyperScript™Ⅳ RT SuperMix for qPCR (with gDNA wiper) is suitable for two-step RT-qPCR assays, 5 × HyperScript™ Ⅳ RT SuperMix contains all components for reverse transcription except the template. The inclusion of the 4× gDNA wiper in the product is intended to remove potential genomic DNA contamination before reverse transcription. This kit is ideal for reverse transcription of low-concentration RNA templates.

This product has been optimized for qPCR, especially the ratio of Oligo (dT)23VN primer /Random primer, the cDNA synthesis can be initiated from all regions of the RNA transcript with the same efficiency, ensuring maximum authenticity and reproducibility of qPCR results. The reverse transcription products can be employed in SYBR Green or probe-based qPCR, you can choose appropriate reagents for gene expression analysis according to your purpose.