HyperScript™ IV First-Strand cDNA Synthesis Kit
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity compared to wild-type. Therefore, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA.
This kit is based on HyperScript™ III Reverse Transcriptase, contains all the components required for the synthesis of first-strand cDNA, and provides primers for cDNA synthesis: Random Primers and Oligo(dT)23VN. Random Primers, Oligo(dT)23VN or gene-specific primers can be employed as reverse transcription primers according to your needs, and the synthesized first-strand cDNA products can be used for subsequent PCR amplification, qPCR reactions or other experiments.
Features of HyperScript™ IV Reverse Transcriptase:
- Significantly improved resistance to a variety of inhibitors that may interfere with cDNA synthesis
- Robust and specific cDNA synthesis for a wide range of samples
- Faster reverse transcription speed and reduced incubation time from >50 minutes to 10 minutes
- Improved processivity compared to third-generation reverse transcriptase
| Components | 20 rxn (20 μL reaction) | 50 rxn (20 μL reaction) | 100 rxn (20 μL reaction) |
| HyperScript™ IV Reverse Transcriptase (200 U/μL) | 20 μL | 50 μL | 100 μL |
| 5x HSⅣ Buffer | 80 μL | 200 μL | 400 μL |
| RNase Inhibitor, Murine (40 U/μL) | 20 μL | 50 μL | 100 μL |
| 10 mM dNTP Mixture | 20 μL | 50 μL | 100 μL |
| RNase Free ddH2O | 1 ml | 1 ml | 2 X 1mL |
| Random Primer (50 μM) | 20 μL | 50 μL | 100 μL |
| Oligo (dT) 23VN (50 μM) | 20 μL | 50 μL | 100 μL |
Store the components at -20℃ for 2 years. | |||