HyperScript™ III Reverse Transcriptase
HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity compared to wild-type MMLV. Therefore, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA.
This product can tolerate higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, the product has an enhanced affinity for templates, making it suitable for reverse transcription of small amounts of template as well as low-copy genes, and can reverse transcribe cDNA up to 12.3 kb for subsequent PCR, qPCR experiments or other experiments.
| Components | 2,000 U | 10,000 U | 40,000 U |
| HyperScript™ III Reverse Transcriptase (200 U/μL) | 10 μL | 50 μL | 200 μL |
| 5x HyperScript™ III First-Strand Buffer | 40 μL | 200 μL | 800 μL |
Store the components at -20℃ for 2 years. | |||