Geneticin, G-418 Sulfate
G418 sulfate, also called Geneticin, belongs to the aminoglycoside class of antibiotics and shares structural similarities with gentamicin B1. This compound serves as a selectable marker in both plant and mammalian cell culture systems. It can select for cells transformed with neomycin resistance gene.
G418 works by inducing premature termination codons during protein translation, effectively blocking polypeptide chain elongation. Cellular resistance is mediated by either the nptII gene in plant cells or the neo gene in mammalian systems, both of which produce an enzyme that modifies and inactivates G418. To establish optimal working conditions, it is needed to perform a kill curve to identify the minimum concentration required to eliminate non-resistant cells.
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 692.71 |
| Cas No. | 108321-42-2 |
| Formula | C20H40N4O10·2H2SO4 |
| Synonyms | Antibiotic G418 |
| Solubility | insoluble in EtOH; insoluble in DMSO; ≥64.6 mg/mL in H2O |
| Chemical Name | 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid |
| SDF | Download SDF |
| Canonical SMILES | CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1, 2]: | |
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Cell lines |
DENV-2-infected BHK cells |
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Preparation method |
This compound is limited soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
1–300 μg/ml, 120 h |
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Applications |
Geneticin (1–300 μg/ml) inhibited many pro- and eukaryotes. Geneticin reduced viability of BHK cells with a CC50 value of 165 ± 5 μg/ml. Geneticin prevented DENV-induced CPE and increased cell viability of infected cells with an EC50 value of ～3 ± 0.4 μg/ml. Geneticin inhibited the yield of viral titers of DENV-2 even after 72 hpi with an EC50 value of 2 ± 0.1 μg/ml and an EC90 value of about 20 ± 2 μg/ml. Geneticin inhibited the number of plaques produced by the virus, and significantly reduced the sizes of the remaining plaques. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7. [2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092. [3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27. |
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| Description | G-418 is an aminoglycoside antibiotic. | |||||
| Targets | 80 S ribosomes | |||||
| IC50 | ||||||
Quality Control & MSDS
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Chemical structure
