Brassinazole

Brassinazole (CAS No.: 224047-41-0) is a synthetic triazole-type small molecule widely used as a selective inhibitor of brassinosteroid biosynthesis in plant research, serving as a valuable chemical probe to dissect brassinosteroid-mediated signaling pathways that regulate plant growth and development. This compound exerts its biological activity primarily through inhibition of key cytochrome P450 enzymes involved in brassinosteroid biosynthesis, including CYP90B, thereby suppressing endogenous hormone production and downstream signaling processes associated with cell elongation, division, and differentiation. In vitro and whole-plant studies demonstrate that Brassinazole induces characteristic phenotypic alterations at low micromolar concentrations, such as dwarfism, reduced hypocotyl elongation, and changes in leaf morphology and pigmentation, effects that can be reversed by exogenous brassinosteroid supplementation, confirming pathway specificity. Its well-defined mechanism and reversible activity make it a robust tool for functional genomics, hormone signaling studies, and phenotypic screening in model systems such as Arabidopsis and cress. In experimental settings, the concentrations or doses used typically vary depending on the biological system and research objectives, supporting its application in mechanistic studies and high-throughput screening approaches aimed at elucidating plant hormone function and identifying modulators of growth-regulatory pathways.