Protease Inhibitor Cocktail(EDTA-Free,100X in DMSO)

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Shamriz O, Simon AJ, et al. "Exogenous interleukin-2 can rescue in-vitro T cell activation and proliferation in patients with a novel capping protein regulator and myosin 1 linker 2 mutation. Clin Exp Immunol." 2020;200(3):215-227. PMID:32201938
- 2. Gao X, Li X, et al. "Preparation, physicochemical characterization, and anti-proliferation of selenium nanoparticles stabilized by Polyporus umbellatus polysaccharide." Int J Biol Macromol. 2020;S0141-8130 (20)30457-8. PMID:32087224
- 3. Cap KC, Jung YJ, et al. "Distinct dual roles of p-Tyr42 RhoA GTPase in tau phosphorylation and ATP citrate lyase activation upon different Aβconcentrations." Redox Biol. 2020 Jan 31:101446. PMID:32046944
- 4. Lu K, Hou W, et al. "Biological evaluation and chemoproteomics reveal potential antibacterial targets of a cajaninstilbene-acid analogue." Eur J Med Chem. 2020 Feb 15;188:112026. PMID:31918072
- 5. Wang J, Luo J, et al. "Increased intracellular Cl(-) concentration by activating FAK promotes airway epithelial BEAS-2B cells proliferation and wound healing." Arch Biochem Biophys. 2019 Dec 12:108225. PMID:31838119
- 6. Chen Y, Li H, et al. "Sesamin suppresses NSCLC cell proliferation and induces apoptosis via Akt/p53 pathway." Toxicol Appl Pharmacol. 2019 Dec 3:114848. PMID:31809756
- 7. Zheng SC, Chang XJ, et al. "A novel RING finger protein CqRNF152-like with self-ubiquitination activity inhibits white spot syndrome virus infection in a crustacean Cherax quadricarinatus." Fish Shellfish Immunol. 2019 Oct 7. pii: S1050-4648(19)30971-4. PMID:31600596
- 8. Reuven N, Adler J, et al. "Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing." Biomolecules. 2019 Oct 8;9(10). PMID:31597252
- 9. Zhaohui Wang, Yi Ma, et al. "CXCR4‐Enriched Nano‐Trap Targeting CXCL12 in Lung for Early Prevention and Enhanced Photodynamic Therapy of Breast Cancer Metastasis." Advanced Functional Materials. 06 September 2019.
- 10. Zhuoyang Fan, Jing He, et al. "Silencing of HMOX1 enhances apoptosis caused by arsenic trioxide in HCC." Int J Clin Exp Med 2019;12(8):9858-9870
- 11. Cheng L, Li J, et al. "N-linoleyltyrosine protects against transient cerebral ischemia in gerbil via CB2 receptor involvement in PI3K/Akt signaling pathway. " Biol Pharm Bull. 2019 Sep 3. PMID:31484847
- 12. Christopher C. Nguyen, Anthony J. Domma, et al. "ER reorganization and intracellular retention of CD58 are functionally independent properties of the human cytomegalovirus ER resident glycoprotein UL148." bioRxiv. 2019 August 27.
- 13. Sun L, Büeler H. "Proteasome inhibition promotes mono-ubiquitination and nuclear translocation of mature (52 kDa) PINK1." Biochem Biophys Res Commun. 2019 Jul 27. pii: S0006-291X(19)31401-9. PMID:31362890
- 14. Li T, Wei Z, et al. "Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway." Molecules. 2019 Jul 17;24(14). pii: E2596. PMID:31319488
- 15. Na-Na He, Ting Wang, et al. "Yiqi Fusheng Decoction reverses cisplatin resistance in colorectal cancer by down-regulating HIF-1α and p-ERK1/2."Drug Combination Therapy. 2019, Vol. 1 Issue (1): 31-39
- 16. Losier TT, Akuma M,et al. "AMPK Promotes Xenophagy through Priming of Autophagic Kinases upon Detection of Bacterial Outer Membrane Vesicles." Cell Rep. 2019 Feb 19;26(8):2150-2165.e5. PMID:30784596
- 17. Li SM, Zhao YQ, et al. "Upregulation of miR-504-3p is associated with favorable prognosis of acute myeloid leukemia and may serve as a tumor suppressor by targeting MTHFD2." Eur Rev Med Pharmacol Sci. 2019 Feb;23(3):1203-1213. PMID:30779090
- 18. Ma F, Chang X, et al. "Streptococcus Suis Serotype 2 Stimulates Neutrophil Extracellular Traps Formation via Activation of p38 MAPK and ERK1/2." Front Immunol. 2018 Dec 7;9:2854. PMID:30581435
- 19. Liu ZB, Tang C, et al. "Increased expression of lncRNA SNHG12 predicts a poor prognosis of nasopharyngeal carcinoma and regulates cell proliferation and metastasis by modulating Notch signal pathway." Cancer Biomark. 2018;23(4):603-613. PMID:30452404
- 20. Lu XY, Zhou FH, et al. "Codonopsis tangshen Oliv. Amelioration Effect on Diabetic Kidney Disease Rats Induced by High Fat Diet Feeding Combined with Streptozotocin." Nat Prod Bioprospect. 2018 Dec;8(6):441-451. PMID:30387083
- 21. Lu Xianyuan,Zou Wei, et al. "Anti-renal fibrosis effect of asperulosidic acid via TGF-β1/smad2/smad3 and NF-κB signaling pathways in a rat model of unilateral ureteral obstruction." Phytomedicine Available online 5 September 2018.
- 22. Michele Renae Plewes. "The Effects of in Vitro Fish Oil and Dietary Fish Meal Supplementation on Microdomains Within Bovine Luteal Cells." University of Northern Colorado.2018.
- 23. Shi G, Zhang H, et al. "Epigenetic silencing of sonic hedgehog elicits antitumor immune response and suppresses tumor growth by inhibiting the hedgehog signaling pathway in metastatic spine tumors in Sprague-Dawley rats." J Cell Biochem. 2018 Nov;119(11):9591-9603. PMID:30191602
- 24. Gao X, Bao S, et al."Fructose-1,6-bisphosphate aldolase of Mycoplasma bovis is a plasminogen-binding adhesin." Microb Pathog. 2018 Aug 22;124:230-237. PMID:30142464
- 25. M.R. Plewes, P.D. Burns. "Effect of fish oil on agonist-induced receptor internalization of the prostaglandin F 2α receptor and cell signaling in bovine luteal cells in vitro." Domestic Animal Endocrinology. 2017 December 12.
- 26. Rana PS, Mudrak NJ, et al. "Phase separation in necrotic cells." Biochem Biophys Res Commun. 2017 Oct 21;492(3):300-303. PMID:28859980
Related Biological Data

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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | |
A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
Stored at -20°C, and stable for at least 12 months. |
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.
This protease inhibitor cocktail contains individual components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, acid proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for mammalian cells and tissue extracts. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.