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Trichostatin A (TSA)

Catalog No.
A8183
HDAC inhibitor
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$198.00
In stock
1mg
$66.00
In stock
5mg
$209.00
In stock
25mg
$770.00
In stock
100mg
$1,980.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Trichostatin A (TSA) is a potent inhibitor of histone deacetylase (HDAC) as well as an antifungal antibiotic with cytostatic and differentiating properties that noncompetivively and reversibly inhibits HDAC, at low nanomolar concentrations, in both cultured mammalian cells and fractionated cell nuclear extracts. It is capable of arresting cells in G1 and G2 phases of the cell cycle, inducing differentiation and reverting the transformed morphology of cells in culture. According to a study investigating the effect of TSA in human breast cancer cell lines, TSA inhibited proliferation of breast carcinoma cell lines (IC50 124.4 ± 120.4 nM), comparing to all cell lines (IC50 2.4 ± 0.5 nM), and resulted in pronounced histone H4 hyperacetylation.

Reference

David M. Vigushin, Simak Ali, Paul E. Pace, Nina Mirsaidi, Kazuhiro Ito, Ian Adcock, and R. Charles Coombes. Trichostatin A is a histone deacetylase inhibitor with potent antitumot activity against breast cancer in vivo. Clin Cancer Res 2001;7:971-976

Product Citation

Chemical Properties

StorageDesiccate at -20°C
M.Wt302.37
Cas No.58880-19-6
FormulaC17H22N2O3
SynonymsTrichostatin A,TSA
Solubilityinsoluble in H2O; ≥15.12 mg/mL in DMSO; ≥16.56 mg/mL in EtOH with ultrasonic
Chemical Name(2E,4E,6R)-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxohepta-2,4-dienamide
SDFDownload SDF
Canonical SMILESCC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment[1]:

Cell lines

Human breast cancer cell line

Preparation method

The solubility of this compound in DMSO is

Reaction Conditions

10 μM TSA solved in growth medium containing 0.1% (v/v) ethanol for 96 h incubation

Applications

TSA inhibited proliferation of eight breast carcinoma cell lines with mean ± SD IC50 of 124.4 ± 120.4 nM (range, 26.4–308.1 nM). TSA treatment resulted in pronounced histone H4 hyperacetylation.

Animal experiment [1]:

Animal models

Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU

Dosage form

500 μg/kg by injection daily for 4 weeks

Applications

TSA had pronounced antitumor activity in vivo. that The antitumor activity of TSA attributabled to induction of differentiation.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

1. Vigushin DM1, Ali S, Pace PE et al. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin Cancer Res. 2001 Apr;7(4):971-6.

Biological Activity

Targets HDAC          
IC50 ~1.8 nM          

Quality Control

Quality Control & MSDS

View current batch:

Chemical structure

Trichostatin A (TSA)

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