TMB substrate solution for ELISA

TMB, namely 3,3',5,5'-Tetramethylbenzidine, is a commonly used substrate for horseradish peroxidase (HRP). Under the catalysis of HRP or other appropriate peroxidases, TMB produces a soluble blue product. After adding a stop solution, the reaction can be measured using a microplate reader. When H?SO? or HCl is used as the stop solution, a yellow product is generated, allowing accurate intensity measurement at 450 nm using a microplate reader. When NaF is used as the stop solution, it exhibits strong light absorption at 650 nm, and the absorbance can be measured between 620–650 nm.

This product utilizes HRP to catalyze TMB color development and is intended for use in ELISA and related experiments. This chromogenic solution can also be used to detect peroxidase levels in samples such as blood or hemoglobin.