TMB substrate solution for Blotting

TMB, namely 3,3',5,5'-Tetramethylbenzidine, is a commonly used substrate for horseradish peroxidase. Under the catalysis of horseradish peroxidase (HRP) or other appropriate peroxidases, TMB produces a blue oxidation product.

This product utilizes HRP to catalyze TMB color development and is intended for use in immunohistochemistry (IHC), in situ hybridization (ISH), or membrane-based color development experiments. This chromogenic solution can also be used for in situ detection of endogenous peroxidase activity in cells or tissues. Upon reaction with HRP-oxidized TMB substrate, the blue reaction product precipitates at the reaction sites and localizes to the membrane surface or tissue samples. When used in Western Blotting, it is recommended to perform stringent blocking and rigorous washing to avoid high background. This product can visualize protein bands in less than one minute. The reaction can be terminated by washing with ddH?O, and the protein bands remain stable after membrane drying, allowing visualization without special equipment. This product is compatible with both PVDF and nitrocellulose (NC) membranes. For IHC applications, aqueous counterstains and mounting media must be used.