Recombinant Human Monocyte Chemotactic Protein-2/CCL8
Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.
Reference
1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31
2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42
3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74
4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92.
| Gene ID | 6355 |
| Accession # | P80075 |
| Alternate Names | HC14, Small-inducible cytokine A8 |
| Source | Escherichia coli. |
| M.Wt | Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence | QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuMCP-2/CCL8 as determined by LAL method.