Live-Dead Bacterial Staining Kit
- 1. Heng Li, Zhiyue Zhang, et al. "Multifunctional Fe3O4@ ZIF-8 Nanoparticles with Antibiosis and Osteogenesis for Treatment of Jaw Osteomyelitis." Pharmaceutics. 2026 Mar 13;18(3):359. PMID: 41900845
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The Live-Dead Bacterial Staining Kit provides an assay for detecting live bacteria and dead bacteria at the same time. The kit contains two dyes, NucGreen and EthD-III. NucGreen is a green nucleic acid dye that can stain both live and dead bacteria; EthD-III is a red nucleic acid dye that only stains bacteria with damaged cell membranes, such as dead bacteria. The viability of bacteria can be identified through the kit, with healthy bacteria appearing green and dead bacteria having a superposition of green and red.

Figure 1: Effect of this kit for detecting bacterial viability
| Components | K2239-20 T | K2239-100 T |
| NucGreen | 20 μL | 100 μL |
| EthD-III | 40 μL | 200 μL |
Store at -20°C away from light, avoiding repeated freeze and thaw cycles, stable for 6 months. | ||
| Shipping | Blue ice. |







