Arachidonoyl thio-PC

Arachidonoyl thio-PC (CAS No.: 146797-82-2) is a synthetic phospholipid analog widely employed as a biochemical substrate for investigating phospholipase A2 (PLA2) enzymatic activity, including secretory (sPLA2), cytosolic (cPLA2), and calcium-independent (iPLA2) isoforms. Upon enzymatic cleavage at the sn-2 position, this substrate releases a free thiol group that readily reacts with chromogenic reagents such as DTNB or DTP, enabling sensitive and quantitative detection of PLA2 activity in vitro. This reaction principle underpins its extensive use in mechanistic enzymology, isozyme-selective activity profiling, and high-throughput screening assays targeting lipid metabolism and inflammatory signaling pathways. By selectively inhibiting or excluding competing PLA2 isoforms, Arachidonoyl thio-PC facilitates the assessment of cPLA2-specific activity, supporting studies of arachidonic acid mobilization and downstream eicosanoid biosynthesis. Its activity is typically evaluated in enzymatic assays within low micromolar to micromolar substrate concentration ranges, although conditions vary depending on assay design and enzyme source. In biomedical research and drug discovery, this compound is commonly applied in cell-free systems and cell-based models to characterize PLA2 function, screen inhibitors, and elucidate the role of phospholipid hydrolysis in inflammation-related diseases and other lipid-associated pathologies.