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Phosphatase Inhibitor Cocktail 1 (100X in DMSO)
For serine/threonine protein phosphatases and L-isozymes of alkaline phosphatases.

Catalog No.K1012
Size Price Stock Qty
In stock
1ml x 10
In stock
1ml x 50
In stock
1ml x 100
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & DataSheet

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Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and storage

Catalog No. Product Name Summary Targets CAS Number Smiles
N1686 Cantharidin Inhibitor of protein phosphatases 1/2A Nature Products 56-25-7 O=C([[email protected]@]1(C([H])([H])[H])[[email protected]]2(C([H])([H])[H])[[email protected]@]3([H])O[[email protected]]1([H])C([H])([H])C3([H])[H])OC2=O
B4750 (-)-p-Bromotetramisole Oxalate ALP inhibitor, potent and non-specific Others|Others 62284-79-1 BrC(C=C1)=CC=C1[[email protected]]2N=C3SCCN3C2.OC(C(O)=O)=O
B3698 Microcystin-LR Inhibitor of protein phosphatase PP1/PP2A,potent and selective Chromatin/Epigenetics|Protein Ser/Thr Phosphatases 101043-37-2 CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C
Stored at -20°C, and stable for at least 12 months. When kept at 2-8°C, the product remains stable for 2 months.


Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading and modifying proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Phosphatase Inhibitor Cocktail 1 inhibits L-isozymes of alkaline phosphatases and serine/threonine protein phosphatases such as protein phosphatases 1 and 2A (PP1 and PP2A). This phosphatase inhibitor cocktail has been optimized and tested on cell extracts from various animal tissues.

This phosphatase inhibitor cocktail contains individual components, including Cantharidin, Bromotetramisole and Microcystin LR. This phosphatase inhibitor cocktail is supplied as a ready-to-use solution in DMSO.


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