In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
UNC1215 is a selective inhibitor of L3MBTL3 with IC50 value of 40 nM .
L3MBTL3 is a member of the MBT (malignant brain tumor) family of methyl-lysine reader proteins and is reported to play an important role in haematopoiesis and cancer biology. And it is reported that inhibition of L3MBTL3 can be regarded as a promising target used in clinic .
UNC1215 is a potent L3MBTL3 inhibitor and has a more potent inhibitory ability than other MBT family members. When tested with HEK293 cells transfected with a GFP fusion protein of the 3 MBT domains of L3MBTL3, UNC1215 treatment decreased the recovery time in a dose responsive manner via binding and co-localizing L3MBTL3 . Using AlphaScreen○R methylated histone peptide competition assay, UNC1215 showed high antagonism ability to L3MBTL3 with IC50 value of 24±7.6 nM . As the first potent and selective inhibitor for methyl-lysine reader protein-L3MBTL3-UNC1215 showed highly selective inhibitor ability via antagonizing the mono- and dimethyl-lysine reading function of L3MBTL3 .
1. James, L.I., et al., Discovery of a chemical probe for the L3MBTL3 methyllysine reader domain. Nat Chem Biol, 2013. 9(3): p. 184-91.
2. James, L.I., et al., Small-molecule ligands of methyl-lysine binding proteins: optimization of selectivity for L3MBTL3. J Med Chem, 2013. 56(18): p. 7358-71.
3. Camerino, M.A., et al., The structure-activity relationships of L3MBTL3 inhibitors: flexibility of the dimer interface. Medchemcomm, 2013. 4(11): p. 1501-1507.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥26.05 mg/mL in DMSO, ≥4.94 mg/mL in EtOH with ultrasonic and warming, <2.61 mg/mL in H2O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Cell experiment :|
Human embryonic kidney 293 cells transfected with a GFP fusion protein of the three MBT domains of L3MBTL3 (GFP-3MBT)
The solubility of this compound in DMSO is >26.05 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
EC50: 50–100 nM
Treatment with UNC1215 decreased the recovery time fluorescence intensity following photobleaching in a dose-responsive manner, with the EC50 of 50–100 nM, indicating that UNC1215 promoted diffusibility of GFP-3MBT within the nucleus. UNC1215 competed with cellular factors for binding of the MBT domains at concentrations well below 1 μM. UNC1215 showed potent effects on the subnuclear localization of GFP-3MBT, with an IC50 of approximately 500 nM for disruption of foci formation.
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
. James L I, Barsyte-Lovejoy D, Zhong N, et al. Discovery of a chemical probe for the L3MBTL3 methyllysine reader domain[J]. Nature chemical biology, 2013, 9(3): 184-191.
|Description||Ko 143 is a potent inhibitor of L3MBTL3 methyllysine (Kme) reader domain with an IC50 value of 40 nM and Kd value of 120 nM.|
|IC50||40 nM (Kd=120 nM)||3.5 μM|