Recombinant Human/Murine/Rat Activin A
Activins and Inhibins are TGF-β superfamily cytokines that are involved in tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. Activin A protects the heart from hypoxic stress and promotes the differentiation of embryonic stem cells into cardiomyocytes. Activins are homodimers or heterodimers of various β subunits (βA, βB, βC, and βE), while Inhibins are heterodimers of a unique α subunit and one of the β subunits. Activin A is a homodimer of two βA chains. Human βA shares 100% amino acid sequence identity with mouse, rat, bovine, porcine, and feline βA. Activin A binds to Activin RIIA which then associates with Activin RIB/ALK-4. Activin A bioactivity is regulated by cell-associated molecules (BAMBI, Betaglycan, and Cripto) and soluble molecules (β2-Macroglobulin, Follistatin, and FLRG).
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Gene ID |
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Accession # |
P08476 |
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Alternate Names |
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Source |
Chinese Hamster Ovary cell line, CHO |
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M.Wt |
Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains. |
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AA Sequence |
Gly311 - Ser426; Accession # P08476 |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from 0.2 μm filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions. |
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Biological Activity |
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Recombinant Human/Murine/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95 % by SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 0.01 EU/µg of rHu/Mu/RtActivin A as determined by LAL method.