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Ouabain

Catalog No.
B2270
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selective Na+/K+-ATPase inhibitor
Grouped product items
SizePriceStock Qty
50mg
$105.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Ouabain is a selective Na+/K+, -ATPase inhibitor, binds to α2 /α3 subunit with Ki of 41 nM/15 nM.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt584.66
Cas No.630-60-4
FormulaC29H44O12
Solubility≥72.9 mg/mL in DMSO
Chemical Name4-((1R,3S,5S,8R,9S,10R,11R,13R,14S,17R)-1,5,11,14-tetrahydroxy-10-(hydroxymethyl)-13-methyl-3-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)hexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)furan-2(5H)-one
SDFDownload SDF
Canonical SMILESC[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O[C@H]2C[C@@H](O)[C@]3(CO)[C@]4([H])[C@@]([C@@]5(O)CC[C@H](C6=CC(OC6)=O)[C@@]5(C)C[C@H]4O)([H])CC[C@]3(O)C2)O1
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

Rat astrocytes

Reaction Conditions

0.1 ~ 1 μM ouabain

Applications

In cultured rat astrocytes, 0.1 ~ 1 μM of ouabain inhibited the Na+ pump and increased stored Ca2+. As an inhibitor of the Na+ pump, ouabain could be used to explore the distribution and function of the isoforms of the Na+ pump catalytic (α) subunit due to its different affinity for each isoform.
Animal experiment:[2]

Animal models

Male Wistar rats with heart failure due to myocardial infarction (MI)

Dosage form

14.4 mg/kg/day

Injected subcutaneously (s.c.) for 2 weeks; either continuously (osmotic minipumps) or intermittently (once daily)

Applications

Ouabain (14.4 mg/kg/day; s.c.; intermittent) further increased total peripheral resistance (TPR) in rats with heart failure due to MI, while continuous ouabain treatment normalized TPR in MI rats. Ouabain (14.4 mg/kg.d; s.c.; continuous) significantly improved basal and maximal CO.

Note

The technical data provided above is for reference only.

References:

1. Juhaszova M, Blaustein MP. Na+ pump low and high ouabain affinity alpha subunit isoforms are differently distributed in cells. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(5): 1800-1805.

2. Nelissen-Vrancken HJ, Wang JF, Struijker Boudier HA, et al. Ouabain improves cardiac function in vivo in rats with heart failure after chronic but not acute treatment. Naunyn-Schmiedeberg's Archives of Pharmacology, 1997, 356(2): 203-209.

Quality Control

Quality Control & MSDS

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Chemical structure

Ouabain