Ouabain
Ouabain (g-strophanthin) is a potent, specific, and cell-impermeable small-molecule inhibitor of Na⁺/K⁺-ATPase. Originally derived from plants, it is a classic research tool for studying ion transport, cellular signaling, and cardiotonic steroid physiology.
Its exclusive high-affinity target is the extracellular α-subunit of the Na⁺/K⁺-ATPase (sodium-potassium pump). By binding to and inhibiting this essential ion transporter, Ouabain disrupts the transmembrane Na⁺ and K⁺ gradient, leading to increased intracellular Na⁺. This, in turn, affects Ca²⁺ homeostasis via the Na⁺/Ca²⁺ exchanger (NCX), a key mechanism underlying its physiological effects.
- 1. Luyun Zhang, Zhiming Zhu, Hui Dong. "Novel mechanisms of metformin-induced vasorelaxation of mesenteric arterioles via endothelium-dependent hyperpolarization to treat murine colitis." Eur J Pharmacol . 2025 Jul 3:1003:177900 PMID: 40617384
- 2. Vanessa Smer-Barreto, Andrea Quintanilla, et al. "Discovery of senolytics using machine learning." Nat Commun. 2023 Jun 10;14(1):3445 PMID: 37301862
| Storage | Store at -20°C |
| M.Wt | 584.66 |
| Cas No. | 630-60-4 |
| Formula | C29H44O12 |
| Solubility | ≥72.9 mg/mL in DMSO |
| Chemical Name | 4-((1R,3S,5S,8R,9S,10R,11R,13R,14S,17R)-1,5,11,14-tetrahydroxy-10-(hydroxymethyl)-13-methyl-3-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)hexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)furan-2(5H)-one |
| Canonical SMILES | C[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](O[C@H]2C[C@@H](O)[C@]3(CO)[C@]4([H])[C@@]([C@@]5(O)CC[C@H](C6=CC(OC6)=O)[C@@]5(C)C[C@H]4O)([H])CC[C@]3(O)C2)O1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Rat astrocytes |
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Reaction Conditions |
0.1 ~ 1 μM ouabain |
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Applications |
In cultured rat astrocytes, 0.1 ~ 1 μM of ouabain inhibited the Na+ pump and increased stored Ca2+. As an inhibitor of the Na+ pump, ouabain could be used to explore the distribution and function of the isoforms of the Na+ pump catalytic (α) subunit due to its different affinity for each isoform. |
| Animal experiment:[2] | |
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Animal models |
Male Wistar rats with heart failure due to myocardial infarction (MI) |
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Dosage form |
14.4 mg/kg/day Injected subcutaneously (s.c.) for 2 weeks; either continuously (osmotic minipumps) or intermittently (once daily) |
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Applications |
Ouabain (14.4 mg/kg/day; s.c.; intermittent) further increased total peripheral resistance (TPR) in rats with heart failure due to MI, while continuous ouabain treatment normalized TPR in MI rats. Ouabain (14.4 mg/kg.d; s.c.; continuous) significantly improved basal and maximal CO. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Juhaszova M, Blaustein MP. Na+ pump low and high ouabain affinity alpha subunit isoforms are differently distributed in cells. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(5): 1800-1805. 2. Nelissen-Vrancken HJ, Wang JF, Struijker Boudier HA, et al. Ouabain improves cardiac function in vivo in rats with heart failure after chronic but not acute treatment. Naunyn-Schmiedeberg's Archives of Pharmacology, 1997, 356(2): 203-209. |
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Quality Control & MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
