Nigericin sodium salt

Nigericin sodium salt (CAS: 28643-80-3) is the sodium salt of nigericin. Nigericin reversibly binds K+ through coordination of the cation by its polyether oxygen atoms, forming a neutral, membrane-permeable complex that diffuses across the lipid bilayer. After releasing K+ on the opposite side of the membrane, nigericin binds H+ and transports it in the reverse direction. Through repeated cycles of K+ binding, transmembrane diffusion, ion exchange, and proton return, nigericin mediates exchange of K+ and H+ across biological membranes without generating a net electrical current. This process rapidly dissipates transmembrane K+ and proton gradients, resulting in intracellular K+ depletion and cytosolic acidification.

In inflammasome research, nigericin is widely used as a potent activator of the NLRP3 inflammasome and a classical inducer of pyroptosis. Following lipopolysaccharide (LPS)-mediated priming, nigericin promotes rapid K+ efflux, which lowers intracellular potassium concentrations below the threshold required to maintain the inactive conformation of NLRP3. The resulting conformational change triggers NLRP3 oligomerization and recruitment of the adaptor protein ASC through pyrin domain (PYD)-PYD interactions. ASC subsequently recruits pro-caspase-1 via caspase recruitment domain (CARD)-CARD interactions, leading to inflammasome assembly and caspase-1 activation. Activated caspase-1 cleaves gasdermin D (GSDMD) to generate its pore-forming N-terminal fragment and processes the precursor forms of interleukin-1β (IL-1β) and interleukin-18 (IL-18) into their mature cytokines. GSDMD-mediated plasma membrane pore formation ultimately results in cell swelling, membrane rupture, cytokine release, and pyroptotic cell death.

In addition to its widespread application in inflammasome studies, nigericin has been extensively used to investigate ion transport and bioenergetics. In Na⁺-containing media, nigericin has little effect on ADP-induced platelet aggregation but only modestly inhibits thrombin-induced platelet aggregation, an effect that has been attributed primarily to nigericin-induced cytosolic acidification rather than alterations in potassium homeostasis. Nigericin has also been reported to function as an efficient ion carrier for Pb2+, and physiological concentrations of Ca2+ or Mg2+ have minimal influence on nigericin-mediated Pb2+ transport, whereas varying concentrations of K+ or Na+ produce only minor effects, suggesting potential applications in studies of lead detoxification. Furthermore, nigericin modulates mitochondrial energy metabolism by inhibiting ATP-driven transhydrogenase activity over a broad range of ATP concentrations, with more pronounced inhibition observed at lower ATP levels, while simultaneously stimulating the carbonyl reduction reaction under saturating ATP conditions.

Reference:

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