Immunol Staining Blocking Solution

Immunol Staining Blocking Buffer is used for blocking sections or cell samples in immunostaining. After blocking, non-specific binding of subsequent primary or secondary antibodies to the sample is reduced, background is lowered, and the signal-to-noise ratio is enhanced.

This immunostaining blocking buffer contains an appropriate amount of Triton X-100, which permeabilizes cell membranes. Cell samples treated with this blocking buffer enable the detection of intracellular target proteins, including those in the cytoplasm and nucleus.

The main active components of this product include an optimally balanced amount of BSA and appropriate detergents. These ingredients effectively block non-specific protein binding sites on the sample while reducing non-specific binding of subsequent primary and secondary antibodies.

This product can be used for diluting primary antibodies, but the use of Immunol Staining Primary Antibody Dilution Buffer (K4655), which is specifically designed for primary antibody dilution in immunostaining, is highly recommended.

Based on a blocking volume of 5–10 mL per set of samples, one 100 mL package of this blocking buffer can block 10–20 sets of samples.