RIPA Lysis Buffer (Strong)

RIPA Lysis Buffer (Strong) is widely used for reporter gene assays, protein kinase experiments, immunoassays, and protein purification. Its core components are 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and common protease and phosphatase inhibitors (Sodium orthovanadate, Sodium fluoride, EDTA), which effectively prevent protein degradation. This lysis buffer does not contain a complete protease and phosphatase inhibitor cocktail. For optimal protein protection, please add a comprehensive protease and phosphatase inhibitor cocktail additionally.

This product is suitable for protein extraction from animal cells and tissues. The obtained protein samples can be used in a variety of routine immunological assays, such as Western blot (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA).

For 100 mL of this product, 150–250 μL of lysis buffer is required per well in a 6?well plate, allowing one bottle to be used for approximately 400–666 wells (samples). For tissue samples, 150–250 μL of lysis buffer is required per 20 mg of tissue, so one bottle can be used for approximately 400–666 extractions. The exact volume can be adjusted according to sample density and lysis efficiency.