HyperLight™ PicoGreen dsDNA Assay Kit

Picogreen is an extremely sensitive fluorescent nucleic acid dye that only emits fluorescence when it binds to double-stranded DNA. The fluorescence produced is proportional to the DNA concentration, exhibiting high sensitivity and strong specificity. The HyperLightTM PicoGreen dsDNA Kit, based on the PicoGreen dye, is a product used for the fluorescence detection and quantification of double-stranded DNA. It is highly sensitive and can withstand high concentrations of salts, urea, detergents, proteins, etc., making it easy to operate. It can be used for high-throughput sample reading and has a wide range of applications. In contrast, traditional DNA content detection methods measure the optical absorption value at 260nm (A260), with the main disadvantage being that nucleotides, single-stranded nucleic acids, and proteins significantly affect the signal, and contaminants can interfere during the nucleic acid preparation process, making it impossible to distinguish between DNA and RNA. The PicoGreen kit can be used for cDNA library construction, purification of cloned DNA fragments, DNA quantification, product amplification, and further detection of primers.