Direct Mouse Genotyping Kit Plus
The Direct Mouse Genotyping Kit Plus is designed for rapid extraction and direct PCR amplification of genomic DNA from mouse tissues. It combines optimized tissue lysis buffer and neutralization agents to release genomic DNA efficiently without requiring further purification or precipitation steps, allowing researchers to directly utilize the lysate as a ready template for PCR reactions. By simplifying the workflow, this kit facilitates routine mouse genotyping assays, transgene detection, gene knockout validation, and animal colony screening in biomedical and genetic research laboratories. Additionally, the kit includes a pre-mixed PCR master solution containing dye reagents, providing convenience and increased accuracy for PCR amplification and gel electrophoresis analyses.
- 1. Xinyu Tang, Qian Chen, et al. "Macrophage EP4 Deficiency Drives Atherosclerosis Progression via CD36-Mediated Lipid Uptake and M1 Polarization." Cells. 2025 Jul 4;14(13):1021 PMID: 40643540
- 2. Han-Ying Huang, Yan-Zhou Chen, et al. "Alternations in inflammatory macrophage niche drive phenotypic and functional plasticity of Kupffer cells." Nat Commun. 2024 Oct 29;15(1):9337. PMID: 39472435
| Components | 200 rxns | 500 rxns |
| Lysis buffer | 20 mL | 50 mL |
| Balance buffer | 20 mL | 50 mL |
| 2X HyperFusion™ High-Fidelity Master Mix (With dye) | 1 mL x 2 | 1 mL x 5 |
| Proteinase K | 200 μL | 500 μL |
1. Lysis buffer and Balance buffer should be stored at 4°C. 2. 2X HyperFusion™ High-Fidelity Master Mix (With dye) and Proteinase K could be stored at -20°C for 1-2 years. | ||
