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Cy5 hydrazide (non-sulfonated)

Catalog No.
A8145
carbonyl-reactive dye
Grouped product items
SizePriceStock Qty
5mg
$210.00
In stock
25mg
$410.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. The dye examples are proteins subjected to oxidative stress, glycoproteins pre-activated by periodate oxidation, and oligonucleotides with aldehyde moieties. Cy5 hydrazide could replace carbonyl-reactive Alexa Fluor 647 and DyLight 649 dyes. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In HL-60 cells, Protein carbonylation induced by Hydrogen peroxide (H2O2) was quantitatively analyzed by using fluorescent dyes of Cy5-hydrazide dye, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence determination [1].

Reference:
[1] Yoshimura, T. ; Harashima, M.; Kurogi, K.; Suiko, M.; Liu, M.-C.; Sakakibara, Y. A novel procedure for the assessment of the antioxidant capacity of food components. Analytical Biochemistry, 2016, 507, 7–12.

Product Citation

Chemical Properties

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt569.61
FormulaC32H42Cl2N4O
Solubility≥48 mg/mL in DMSO
SDFDownload SDF
Canonical SMILESCC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(N[NH3+])=O)C4=CC=CC=C4C3(C)C
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Spectral properties

Excitation max (nm) 646 Emission max (nm) 662
Extinction Coefficient (M-1cm-1) 250000 Quantum Yield 0.2
CF260 0.03 CF280 0.04

Quality Control

Chemical structure

Sanguinarine chloride

Related Biological Data

Sanguinarine chloride