Celestine Blue Hematoxylin Stain Kit

Plain alum hematoxylin has a major drawback after nuclear staining in that it is relatively sensitive to subsequent acidic dyes, which is common in Van Gieson staining and Masson trichrome staining. During Van Gieson staining, the use of picric acid-fuchsin often causes most of the hematoxylin color to be washed out, making the nuclei barely distinguishable. To address this issue, iron hematoxylin can be used in combination with Celestine Blue, as Celestine Blue is acid-resistant. Moreover, the ferric salts in the Celestine Blue solution can enhance the binding force of alum hematoxylin to the nucleus, resulting in deeper nuclear staining.