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Calpain Activity Fluorometric Assay Kit

Catalog No.
K2062
Exclusively detects activated calpain
Grouped product items
SizePriceStock Qty
100 assays
$498.00
In stock

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Email: [email protected]

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Background

Apoptosis is one of the biological processes that activation of calpain is implicated in. Cell membrane and Ca2+ is required for calpainactivation. The activated calpain is entered into cytosol. The Calpain Activity Assay Kit is a convenient way of measuring calpain activity with optimized buffers and reagents. The Extraction Buffer extracts cytosolic proteins with contaminations from cell membrane and lysosome proteases. It also protects the cell from atuo-activation of calpain during extraction process. Thus, the kit only detects activated calpain in cytosol following treatment of cells with chemicals or drugs inducers. The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence.

Features & Properties

FeaturesSimple one-step procedure; takes only 2 hours. Fast and convenient. The kit contains both positive and negative controls. Active calpain and calpain inhibitor and antibodies are also available separately.
ShippingDry ice
Storage ConditionsStore at -70°C.

Quality Control

Quality Control & DataSheet

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Related Biological Data

Calpain Activity Fluorometric Assay Kit

Related Biological Data

Calpain Activity Fluorometric Assay Kit

Related Biological Data

Calpain Activity Fluorometric Assay Kit
 

Overview

Kit componentsExtraction Buffer 10X Reaction Buffer Calpain Substrate Ac-LLY-AFC Active Calpain I (Positive Control) Calpain Inhibitor Z-LLY-FMK