C-8 Ceramide-1-phosphate

C-8 Ceramide-1-phosphate (CAS No.: 158983-53-0) is a bioactive sphingolipid metabolite widely utilized in biochemical and cell biology research to investigate lipid-mediated signal transduction and cellular homeostasis. As a phosphorylated derivative of ceramide, it functions as a key regulator of intracellular signaling pathways by inhibiting acidic sphingomyelinase activity while concurrently activating phosphatidylinositol 3-kinase (PI3K), leading to increased production of phosphatidylinositol-3,4,5-trisphosphate (PIP3); the accumulation of PIP3 further reinforces suppression of sphingomyelinase, establishing a feedback regulatory loop. Through reversible interconversion with ceramide via kinase- and phosphatase-mediated processes, C-8 Ceramide-1-phosphate contributes to the dynamic balance between pro-apoptotic and pro-survival lipid signals, thereby influencing cellular processes such as apoptosis, proliferation, and DNA synthesis. This compound is commonly applied in in vitro cellular models to study mechanisms governing cell survival signaling, membrane lipid dynamics, and stress responses, with observed activity typically occurring in the nanomolar to low micromolar concentration range depending on the experimental system. It is also employed in pathway-focused studies and screening platforms aimed at elucidating sphingolipid-regulated signaling networks, where dosing and exposure conditions are adjusted according to specific assay requirements and research objectives.