Anhydrotetracycline (hydrochloride)

Anhydrotetracycline hydrochloride (ATC;CAS 13803-65-1) is an effector molecule utilized in tetracycline-controlled gene expression systems, modulating the DNA-binding activity of tetracycline repressor protein (TetR) and its engineered variant reverse TetR (revTetR). TetR binds to operator DNA sequences, inhibiting transcription. ATC binding induces TetR conformational changes, dissociating it from operator DNA, enabling gene expression. Conversely, ATC acts as a corepressor in revTetR systems, enhancing its DNA-binding inhibition. ATC displays approximately 35-fold greater affinity towards TetR compared to tetracycline, with an IC50 near 0.4 μM, employed in inducible gene expression studies in biological research contexts.

References:
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