YAP-TEAD Inhibitor 1 (Peptide 17)

YAP-TEAD-IN-1 (CAS No.: 1659305-78-8) is a synthetic 17-mer peptide tool compound (also known as Peptide 17), classified as a competitive inhibitor targeting protein–protein interactions, used for precise intervention and mechanistic analysis of the YAP–TEAD complex in the Hippo signaling pathway in the context of biomedical research and drug discovery; by binding to TEAD1 with high affinity and displacing YAP, it directly modulates YAP-dependent transcriptional programs and enables functional validation of target engagement. Based on reported biophysical and biochemical data, YAP-TEAD-IN-1 shows stronger binding affinity for TEAD1 than the reference fragment YAP(50–171), with a measured dissociation constant in the low nanomolar range, for example (approximately 15 nM for YAP-TEAD-IN-1, versus approximately 40 nM for the reference fragment), and demonstrates an IC50 of approximately 25 nM in in vitro inhibition assays, with overall activity in the low nanomolar range. This compound is commonly used in biochemical binding and competition experiments, in vitro assays such as thermal shift or isothermal titration, and cell-level reporter gene and gene expression analyses to validate target binding, map Hippo pathway dependency, and serve as a positive control in discovery workflows focused on oncogenic signaling research; in more complex research systems (such as pathway studies in animal models and candidate molecule evaluation), it may also be used for mechanistic validation and construction of key readouts. The concentrations used in experiments typically depend on cell type, assay format, exposure duration, and delivery strategy, covering the nanomolar to micromolar range. This material is for laboratory research use only and is not intended for any clinical, healthcare, dietary, or other non-research purposes.