Western Blot Transfer Buffer (Standard, Powder)

Western blot transfer buffer is a core reagent used in Western blot experiments to transfer proteins from polyacrylamide gels to solid-phase membranes (such as PVDF or nitrocellulose membranes). Its working principle is based on electroblotting technology: under the influence of an electric field, negatively charged proteins migrate out of the gel and are adsorbed onto the surface of the solid-phase membrane, forming a "blot" that faithfully reflects the protein distribution pattern within the gel.

This product is a standard basic-type transfer buffer, suitable for routine protein transfer. Compared with rapid transfer buffer (wet transfer time ~20–25 min, 400 mA) and ice-free rapid transfer buffer (wet transfer time ~15–40 min, 400 mA, minimal temperature rise during transfer, no ice bath required), this product requires longer transfer time under conventional conditions, but offers milder conditions and broader applicability. It is recommended to perform wet transfer at 4°C to minimize protein degradation and band diffusion.

This product features the classic Towbin formulation (Tris-Glycine system) and is supplied as a ready-to-use powder, free of methanol and SDS. Each pouch prepares 1 L of 1× working solution (200 mL methanol needs to be added before use) and remains stable for 2 years when stored at room temperature.