Western Blot Transfer Buffer (Rapid, Powder)
Western blot transfer buffer is a core reagent used in Western blot experiments to transfer proteins from polyacrylamide gels to solid-phase membranes (such as PVDF or nitrocellulose membranes). Its working principle is based on electroblotting technology: under the influence of an electric field, negatively charged proteins migrate out of the gel and are adsorbed onto the surface of the solid-phase membrane, forming a "blot" that faithfully reflects the protein distribution pattern within the gel.
This product is a rapid-type transfer buffer, compatible with Tris-Glycine and Bis-Tris transfer buffer systems. It significantly shortens wet transfer time while maintaining high transfer efficiency, and the cooling ions in the buffer effectively reduce heat generation during the electrotransfer process, making it suitable for routine and medium-to-high-throughput wet transfer applications. Distinguished from standard transfer buffers (transfer time 60–120 min, 200–300 mA, ice-bath required) and ice-free rapid transfer buffers (transfer time approximately 15–40 min, 400 mA, minimal temperature rise during transfer, no cooling measures required), this product enables rapid transfer at room temperature in 20–25 min (at constant current 400 mA), striking an optimal balance between speed and operational convenience, and is effective for simultaneous transfer of proteins ranging from 10 to 245 kDa.
This product is supplied as a ready-to-use powder formulation, featuring an optimized Tris-Glycine system, free of SDS and methanol (anhydrous ethanol is used as a substitute during preparation). Each pouch prepares 1 L of 1× working solution and remains stable for 2 years when stored at room temperature.
Simple operation: Dissolve the powder in water and mix with ethanol.
Fast: Complete wet transfer within 20–25 min.
Low heat: Significantly less heat than conventional buffers.
Efficient: Pre-stained markers fully transferred in just 20 min.
Broad range: Transfers 10–245 kDa proteins simultaneously.