Western Blot Transfer Buffer (Rapid, Ice‑Free, Powder)

Western blot transfer buffer is a core reagent used in Western blot experiments to transfer proteins from polyacrylamide gels to solid-phase membranes (such as PVDF or nitrocellulose membranes). Its working principle is based on electroblotting technology: under the influence of an electric field, negatively charged proteins migrate out of the gel and are adsorbed onto the surface of the solid-phase membrane, forming a "blot" that faithfully reflects the protein distribution pattern within the gel.

This product is an ice-free rapid-type transfer buffer, compatible with Tris-Glycine and Bis-Tris transfer buffer systems. Through a proprietary formulation, it significantly reduces heat generation during wet transfer, enabling efficient transfer without the need for an ice bath. Distinguished from standard transfer buffers (transfer time 60–120 min, 200–300 mA, ice-bath required) and rapid transfer buffers (transfer time 20–25 min, 400 mA, ice-bath/room temperature), this product enables wet transfer at room temperature in 15–40 min (at 400 mA), with minimal temperature rise during the process and no cooling measures required. It is the most convenient and least demanding model among the three, particularly suitable for laboratories without ice-bath equipment or for high-throughput continuous transfer applications. This product offers broad applicability, with transfer time flexibly adjustable (15–40 min) according to gel concentration. Under recommended conditions (400 mA, 1.0 mm 6% gel), proteins below 150 kDa can be completely transferred, while proteins >150 kDa require only 5–10 min of extended transfer time.

This product is supplied as a ready-to-use powder formulation. Anhydrous ethanol is used as a substitute for methanol during preparation, avoiding the use of toxic reagents. Each pouch prepares 1 L of 1× working solution and remains stable for 12 months when stored at room temperature.

Efficient: 15–40 min wet transfer, equal or superior to conventional.

Cool: Minimal heat, no ice bath needed.

Simple: Just mix buffer + ethanol + water.

Safe: Ethanol replaces methanol—no toxicity.