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Trypsin Activity Colorimetric Assay Kit

Catalog No.
K2176
Sensitive, Colorimetric Assay
Grouped product items
SizePriceStock Qty
100 assays
$1,075.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Trypsin is a serine protease that hydrolyses proteins in the digestive system of various vertebrates. Pancreas produces trypsin as inactive proenzyme trypsinogen. Active trypsin cleaves peptide chains predominantly at the carboxyl side of the amino acids (lysine or arginine).  In the Trypsin Activity Colorimetric Assay, trypsin cleaves a substrate to produce p-nitroaniline (p-NA) that is detectable at λ = 405 nm. Trypsin activity can be measured as the color intensity is proportional to p-NA content. The kit detection limit is 10-100 mU (p-NA unit) trypsin in different samples.

Features & Properties

FeaturesSimple procedure; Takes approx. 1-2 hours based on the levels of trypsin Fast and convenient.
ShippingBlue Ice
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsTrypsin Assay Buffer, Trypsin Substrate (in DMSO), p-NA Standard (2 mM), Trypsin Inhibitor (TLCK, 20 mM), Chymotrypsin Inhibitor (TPCK, 10 mM),