Treosulfan

Treosulfan (CAS No.: 299-75-2) is a bifunctional alkylating agent widely used in biomedical research and drug development, primarily to investigate its potential in antitumor therapy. Its mechanism of action involves forming covalent bonds with DNA, leading to DNA strand breaks and crosslinking, thereby inhibiting cancer cell proliferation and inducing apoptosis. In vitro studies show that Treosulfan exhibits nearly 100% cytotoxicity against multiple cancer cell lines (such as Panc-1, Miapaca-2, and Capan-2) at a concentration of 100 μg/mL, with IC50 values of 3.6 μg/mL, 1.8 μg/mL, and 2.1 μg/mL, respectively, indicating significant anticancer activity at low micromolar levels. In cell models, Treosulfan is commonly used to evaluate its effects on tumor cell proliferation and apoptosis, and the doses or concentrations used in experiments typically depend on the experimental design and research objectives. In animal models, Treosulfan has been used to study its effects; for example, in mouse models, Treosulfan can cause rapid myeloablation and affect the numbers of splenic B and T cells. These studies provide important experimental evidence for the application of Treosulfan in antitumor therapy.