Tn5 Transposase

Tn5 Transposase is a hyperactive mutant of the wild-type Tn5 transposase, which can efficiently insert Tn5 transposons into target sequences in vitro. Tn5 Transposase specifically recognizes the mosaic end (ME) sequences, which are inverted repeats at both ends of the Tn5 transposon. The in vitro transposition insertion efficiency of Tn5 Transposase is 1,000-fold higher than that of wild-type Tn5 transposase.

The insertion sites of Tn5 Transposase are highly random, enabling random integration of double-stranded DNA fragments containing paired recognition sequences into the genome of host cells (in vitro transgenesis). In addition, Tn5 Transposase is widely used in research fields such as next-generation sequencing (NGS) library preparation.