Thidiazuron

Thidiazuron (CAS No.: 51707-55-2) is a phenylurea-derived plant growth regulator with potent cytokinin-like activity that functions as a biochemical reagent widely employed in life sciences research, particularly in studies of plant cell biology and regenerative processes. Acting as a functional analog of endogenous cytokinins, thidiazuron is known to interact with cytokinin receptors and activate downstream signaling pathways that regulate cell division, dedifferentiation, organogenesis, and morphogenesis, thereby promoting callus formation and in vitro plant regeneration. It has demonstrated robust activity across nanomolar to micromolar ranges in plant tissue systems, including stimulation of callus proliferation and induction of shoot regeneration, making it a valuable tool for elucidating mechanisms of plant cell growth, differentiation, and developmental plasticity. Beyond plant systems, thidiazuron has shown notable bioactivity in mammalian cell models, where it modulates epithelial–mesenchymal transition-associated markers and induces apoptosis in certain cancer cell lines at micromolar concentrations, suggesting potential utility in exploratory oncology research. Its applications extend to the development of plant-based bioproduction platforms and experimental models for studying cellular reprogramming and signal transduction, with concentrations and treatment regimens typically optimized according to specific experimental objectives.