TF-1 Cells
TF-1 cells are isolated from a heparin anticoagulant bone marrow aspiration sample from a 35-year-old Japanese man with severe pancytopenia in 1987. The growth of TF-1 cells is completely dependent on IL-3 or GM-CSF, but does not respond to IL-5. In addition, TF-1 cells respond to a variety of lymphokines and cytokines, including IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, CSF, LIF, and NGF.
Cell line |
TF-1 |
Alias |
TF1, MFD-1 |
Organism |
Human sapiens |
Tissue |
Bone, marrow |
Product format |
1 x 106 Cells/Frozen vial |
Cell morphology |
lymphoblast |
Growth properties |
Suspension growth |
Culture medium |
RPMI-1640+2 ng/mL recombinant human GM-CSF+10%FBS+1% P/S |
Culture conditions |
95% air, 5% CO2, 37°C |
Passage ratio |
1:2-1:4 |
Cryopreservation |
90%FBS+10%DMSO |