TCPOBOP

TCPOBOP (CAS No.: 76150-91-9) is a potent and selective agonist of the constitutive androstane receptor (CAR), widely utilized as a chemical probe in hepatic biology and xenobiotic metabolism research. It exhibits strong species specificity, robustly activating mouse CAR at nanomolar potency while showing minimal activity toward human or rat CAR, making it particularly valuable in murine models. Upon CAR activation, TCPOBOP drives transcriptional programs that induce cytochrome P450 monooxygenases, multidrug resistance proteins, and xenobiotic efflux transporters, thereby modulating hepatic detoxification pathways and metabolic homeostasis. Functionally, it promotes pronounced hepatocyte proliferation and liver enlargement without overt tissue injury, and has been shown to attenuate Fas-mediated liver damage in mice through regulation of apoptosis-associated proteins such as Bcl-2. These properties position TCPOBOP as an important tool for studying liver regeneration, hepatomegaly, nuclear receptor signaling, and gene–environment interactions in toxicology and pharmacology. Its activity can be modulated by retinoic acids, highlighting regulatory crosstalk within nuclear receptor networks. In experimental settings, TCPOBOP is commonly applied in cell-based assays and in vivo mouse studies, with concentrations or dosing regimens adjusted according to specific study designs to investigate CAR-dependent gene expression, metabolic enzyme induction, and liver pathophysiology.