T4 RNA Ligase 2 (dsRNA Ligase)
T4 RNA Ligase 2 is an enzyme that catalyzes the formation of a phosphodiester bond between the 5'-phosphorylated terminus and the 3'-hydroxyl terminus of an RNA strand, either intramolecularly or intermolecularly, within double-stranded RNA (dsRNA), double-stranded DNA (dsDNA), or RNA/DNA hybrid molecules. Its activity is ATP-dependent. Unlike T4 RNA Ligase 1 (Cat. No. K3151), it exhibits significantly higher ligation activity toward nicks in dsRNA than toward the termini of single-stranded RNA (ssRNA).
Ligation by T4 RNA Ligase 2 requires the presence of a 5'-phosphate and a 3'-hydroxyl group. The ligation reaction can occur between the 5'-phosphate of an RNA or DNA strand and the 3'-hydroxyl of an RNA strand. It is mainly used for intramolecular circularization of dsRNA, nick ligation (i.e., sticky-end ligation of dsRNA), and nick ligation between an RNA 3'-hydroxyl and a DNA 5'-phosphate in double-stranded nucleic acid structures.
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Components |
500 U |
1000 U |
5000 U |
Storage |
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T4 RNA Ligase 2 (10 U/μL) |
50 μL |
100 μL |
500 μL |
-20°C |
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10× T4 RL2 Reaction Buffer |
100 μL |
200 μL |
1 mL |
-20°C |
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PEG8000 (50%) |
200 μL |
400 μL |
2 mL |
-20°C |
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MgCl2 (100 mM) |
100 μL |
200 μL |
1 mL |
-20°C |
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Shipping: Dry Ice Shelf life: 12 months |
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