T4 Endonuclease Ⅶ
Catalog No.
K1105
An endonuclease that degrades mismatched bases, heterologous double-stranded loops, branching DNA, etc
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T4 Endonuclease Ⅶ is a product of T4 phage gene 49 with a molecular weight of about 18 kDa. T4 endonuclease VII is involved in DNA packaging, gene recombination, and mismatch repair in vivo. It has also been shown to degrade mismatched single-base, heterologous double-stranded loops and branched DNA, such as four-way Holliday structures and three-way Y structures in vitro. This product can be used to detect mutants formed by CRISPR/Cas9, TALEN and other gene editing tools, as well as to detect gene point mutations and SNP.
| Components | 500 U | 1000 U | 2500 U |
| T4 Endonuclease Ⅶ (10 U/μL) | 0.05 mL | 0.1 mL | 0.25 mL |
| 10X T4 Endonuclease Ⅶ Reaction Buffer | 0.5 mL | 1 mL | 2.5 mL |
Store the components at -20 °C. | |||