Streptavidin-HyperFluor™ 555

Streptavidin is a 52,800 dalton tetrameric protein that binds to Biotin with high specificity, and one molecule Streptavidin can bind to 4 Biotin molecules, and this binding is irreversible. Compared with avidin, the affinity of Streptavidin and Avidin, which is derived from egg white, is highly similar to that of Biotin, but Streptividin is almost uncharged under neutral conditions, so the non-specific binding of Streptavidin is much lower than that of Avidin, and the non-specific background of the detection is much less. Streptavidin can be used for the detection of Biotin-labeled antibodies, nucleic acids, proteins, or other biotinylated molecules.

HyperFluorTM 555 is a commonly used fluorescent dye that is widely used in biological and medical research, particularly in techniques such as fluorescence microscopy, flow cytometry, and fluorescence resonance energy transfer (FRET). It emits green fluorescence (yellowish) with an excitation wavelength of about 555 nm and an emission wavelength of about 565 nm.

Streptavidin-HyperFluorTM 555 is a bioprobe that combines Streptavidin protein and HyperFluorTM 555 fluorescent dye, making it a powerful and sensitive fluorescent labeling tool.